Abstract
In the yellow and colorless mutant of C. kesseleri, grown with nitrate as the sole nitrogen source, the addition of glucose or the irradiation of blue light inhibited the uptakes of the nitrate and amino acids in growing cells, while these enhanced the uptake of nitrate and amino acids in starved cells. The addition of methionine sulphoximine (MSX), a potent inhibitor of glutamine synthetase, to growing cells resulted in intracellular ammonium-accumulation and inhibition of uptakes nitrate and amino acids. For the colorless mutant, blue light was shown to activate purified nitrate reductase. These results indicate that in the mutant cells of Chlorella examined, uptake of nitrogenous compounds seems to be influenced by the release of ammonia occurred in the presence of glucose or under blue light.
