Abstract
Classical microsurgical experiments in past have been suggested the importance of meristem activity and signal molecules between meristem and organ primodium. Recently, many key regulators are identified and their expression patterns are analyzed. But such a genetical approach is not enough to understand the timing and pass way of signal transduction in meristematic zone. To elucidate the followed mechanism, it is indispensable to do specific cell ablation in the developing tissue. In tomato, laser ablation system is established, but it is limited because of the shortage of expression maker lines. In this study, we tried to establish UV laser ablation system in shoot apical meristem of Arabidopsis. In present we are succeeded in demolishing the GFP signal of meristematic expression marker. Here, we will discuss about the effect of laser ablation in organ development.
