Abstract
In dicot plants, leaf primodia are derived from the shoot apical meristem and develop along proximal-distal, adaxial-abaxial, and medial-lateral axes. The asymmetric leaves2 (as2) mutant of Arabidopsis thaliana displays pleiotropic phenotypes in leaves, such as downward curling of leaves, reduced complexity of leaf venation pattern, generation of lobes and defect in the leaf adaxial -abaxial polarity. #27 was isolated as an enhancer of as2 mutant. #27 as2-1 mutants had filamentous-like structures that indicate #27 mutant might enhance the defect of adaxial cell differentiation. We analyzed the venation patterns in cotyledons and leaves of wild type, as2-1, #27, and #27 as2-1. RNA-DEPENENT RNA POLYMERASE6 (RDR6) is involved in synthesis of tasiR-ARF and rdr6 was also identified as an enhancer of as2. ETTIN and ARF4 specify abaxial identity and are direct targets of tasiR-ARF mediated mRNA degradation. We will report the genetic analysis of #27 and rdr6.
