Abstract
Monocot plants produce numerous adventitious (crown) roots that are dominant in root system of cereals. We previously reported CRL1 that encodes AS2/LOB, a positive regulator for crown root formation, and is directly regulated by ARF in the auxin signaling pathway. Recently, we isolated CRL5 involved in crown root initiation that encodes the protein highly homologous with AINTEGUMENTA in Arabidopsis and found its expression is induced by auxin. However, it is suggested these genes do not work in the same regulatory pathway of crown root formation by the phenotype of crl1 crl5 double mutants and their expression in each mutant back ground. Then, we tried microarray analysis using crl1 and crl5 mutant to find the candidate genes working in downstream of each CRL. Here, we report the functional analysis of CRL5 through phenotyping of its over expresser and the expression analysis of the candidates working in downstream of each CRL.
