Plant and Cell Physiology Supplement
Abstract of the Annual Meeting of JSPP 2009
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Transcriptional Differences in Male Strobili between the Male-Sterile Mutant and Wild Type of Cryptomeria japonica
*Norihiro FutamuraMaki SaitoKenji Shinohara
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Pages 0672

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Abstract
Sugi (Cryptomeria japonica) is one of the most commercially important conifers but its pollinosis is the most serious allergic disease in Japan. Recently, male-sterile mutants of C. japonica whose pollen is hardly released have been selected. To clarify the molecular mechanisms of male sterility in C. japonica, we have constructed a DNA microarray using clustered ESTs. This microarray was used to analyze gene expression in male strobili of the male-sterile mutant and wild type. There were about 20 ESTs exhibiting differential expression (4-fold changes) at stages before collapsed microspores were observed in the male-sterile mutant. In contrast, over 100 ESTs showed differential expression in male strobili at the stages of microspore, which were collapsed in the male-sterile mutant.
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© 2009 by The Japanese Society of Plant Physiologists
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