GC/MS analysis and Molecular genetical analysis for biosynthesis of endogenous 9-hydroxy-10-oxo-12(Z), 15(Z)- octadecandienoic acid (KODA) in Arabidopsis.

Abstract

Oxylipin, 9-hydroxy-10-oxo-(Z), 15(Z)- octadecadienoic acid (KODA) is one of signal compounds, which is synthesized from the α- linolenic acid. It has been suspected that has a function not only to enhance stress tolerance but also to activate inflorescence development. In biosynthesis in KODA and Jasmonate (JA) it has been suspected that two kinds of enzymes (LOX, AOS) were shared. And some differences in substrate specificity of enzyme activity (9 or 13 oxidation) has also suggested. In Arabidopsis thaliana the expression of one AOS and 6 LOXs were detected, but their involvement in KODA biosynthesis and enzyme activity remained to be elucidated. To clear the correlation between the phenotype of the mutants of LOXs and the levels of endogenous KODA, we established GC/MS analysis method. The differences in these mutants were indicated in phenotype of leaf or inflorescence and the levels of endogenous oxylipines.