Abstract
Jasmonic acid (JA) is a plany hormone that has multiple important roles, such as anther dehiscence at anthesis and resistance to pathogen infection and insect wounding. We have identified Arabidopsis DAD1,which encodes a lipase catalyzing the initial step of JA biosynthesis. Arabidopsis has six homologs of DAD1, namely DAD1-LIKE LIPASE1(DAL1) to DAL6,which encode lipses with transit peptide at their N-terminals. Our studies with multiple mutants showed that DALs also required for JA biosynthesis. To reveal the functional differences among them, we analyzed their expressions with transformants containing promoter-GUS genes. To date, we have examined the expressions of DAL1,2,5,and 6. These four genes were induced by wounding in rosette leaves, but in different cells each other. DAL2 was expressed in stamen filaments as similar to DAD1, while DAL1 and 6 were expressed in other floral organs. DALs might have individual functions in JA biosynthesis.
