Abstract
The Arabidopsis genome is predicted to encode about 20 proteins homologous to the yeast or mammal peroxisome biogenesis factors (peroxin), but their functions remain unclear. In this study, we generated transgenic plants that were suppressed each peroxin gene expression using RNAi method and analyzed whether these homologues actually function for peroxisome biogenesis or not. The transgenic plants that stably expressed GFP-PTS1 were generated as a parent plant for RNAi plant, and we therefore could observe peroxisomes by GFP fluorescent. In the results, we could classified two groups of Arabidopsis peroxin, one group is that GFP is localized in the cytosol and the other defect on peroxisome morphology. Interestingly, PEX10 knock-down plant phenotype is different from wild type plant whereas other peroxin knock-down plants have similar phenotype of wild type plant. Then, we analyzed comprehensive gene expression in PEX10 knock-down plant and discuss PEX10 specific function.
