Abstract
Most of proteins functioning in organelle are encoded by nuclear genes. It is accepted that these genes were translocated from the endsymbiotic bacteria and acquired organelle targeting sequence. However the acquiring process is unknown.
We previously propose a hypothesis that the genes inserted to nuclear genome by horizontal transfer tend to acquire additional sequence to 5' terminal, lead to the acquisition of cryptic organelle targeting sequences.
In this presentation, we reported that N-terminal additional sequences by horizontal transfer to nuclear genome tend to be disordered region. This bias of disordered region was common to N-terminal additional sequences and organelle targeting sequences.
Although disordered regions were unstructured, intrinsic protein disorder is considered to play an important role in protein-protein interaction. Thus horizontal transfer to nuclear genome frequently donates flexible N-terminal sequence to transfer gene. It is expected that a parts of these flexible N-terminal sequence were used as organelle targeting sequences.
