Plant and Cell Physiology Supplement
Abstract of the Annual Meeting of JSPP 2009
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ANALYSIS OF PROPYZAMIDE HYPERSENSITIVE2 (PHS2), RELATED WITH MICROTUBULE REGULATION IN ARABIDOPSIS
*Takehide KatoKuniko NaoiTakashi Hashimoto
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Pages 0740

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Abstract
In growing plant cell, cortical microtubules play an important role in regulating the direction of cell expansion. The phs2 is recessive mutant, which shows slightly left-skewing of root growth under a usual MS condition. But in the presence of 3uM propyzamide, the root growth shows right-skewing. The phs2 mutant had a frame-shift mutation in a novel gene. The genomic fragments including the loci rescued the phs2 drug responses, resulting that the loci was identified as the PHS2 gene. The PHS2 is encoded a TPR motif protein. The four homologous genes exist in Arabidopsis. We constructed GFP-PHS2 transgenic plants using genomic PHS2 clone. Their plants rescued the phs2 phenotype, showing that the GFP-PHS2 protein is functional. The fluorescent were detected on cortical microtubule fibers in the root, hypocotyl and leaf epidermal cells. These results were suggested that PHS2 is a novel microtubule associate protein, which regulates cortical microtubule array in plant.
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© 2009 by The Japanese Society of Plant Physiologists
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