Abstract
Chimeric REpressor gene-Silencing Technology (CRES-T) is a novel gene-silencing technology, in which a transcription factor fused the repression domain (SRDX) is expressed in plant. We had succeeded to suppress the function of various transcription factors by expression of their chimeric repressors under the CaMV35S promoter. However, when CaMV35S promoter is used, the obtained phenotype is occasionally unexpected and may not reflect the original function of transcription factors. In this study, we have developed new CRES-T vectors using MultiSite Gateway system, allowing us to insert a promoter and a gene in various combinations into them. The developed binary vector contains SRDX and Nos terminator downstream of attR4-attR2 Gateway cassette (Nakagawa et al., 2008). We are now analyzing the efficiency of this vector in Arabidopsis.
