Abstract
Cryptomeria japonica is one of the most important Japanese conifer species. For the purpose of creating male sterile GM C. japonica, we attempted to identify the genes related to male flower formation and development.
To identify genes expressed preferentially during male flower development, male flower specific suppression subtractive hybridization (SSH) cDNA libraries were derived from three different stages of male flower development. We isolated seven cDNAs that are differentially expressed in C. japonica male flowers. We have isolated promoters of male flower specific genes using TAIL-PCR method. Those promoters were fused to the GUS gene and transformed into Arabidopsis. Now we are trying to analyze the function of those promoters by histochemical detection of GUS activity in transgenic Arabidopsis.
Acknowledgements: This work was supported in part by AFFRC, MAFF, Japan
