Abstract
Chloroplasts of higher plant have own genomes and its gene expression systems, and major gene expression is regulated by translation level. Therefore understanding of translation regulation mechanisms is essential.
It has been regarded that translation is regulated by 5' untranslated region (5' UTR) which is upstream sequence of coding region on mRNA. But, in practice, it is not always true that strong 5' UTR which binds to any coding regions show strong translation activity. It is regarded that coordination with 5' UTR and coding region especially around sequences of the start codon is important.
In this study, we performed chloroplast in vitro translation experiments using one of the strongest translation activity psbA mRNA in tobacco chloroplasts, and assayed the effects of adjacent region of start codon on translation.
