Abstract
The cysteine protease which has a thiol group in the active center participates in the protein maturation and degradation process in response to the external stimuli, and in various physiological processes such as germination, morphogenesis, and programmed cell death. It is becoming clearer that they are involved in perceiving both biotic and abiotic stresses, signaling and execution
Actinidine of Kiwifruit belongs to a papain family, and the crystal structure is known, and there is also a report that it consists of the isozymes with different pI.
1. We isolated three cysteine proteases with different activity by anion ion exchange chromatography from Actindia (peaks 1, 2 and 3). Until now the crystal structure of peaks 1 and 2 was clarified.
2. Gene amplification was carried out by genomic PCR and RT-PCR by using primers based on the actinidine gene of a kiwi fruit. The clone with the DNA sequence corresponding to the exons has been amplified by RT-PCR.
