Abstract
We established a system in which anthocyanin synthesis of regenerated torenia shoots derived from the leaf discs in vitro was induced on the medium containing high concentration of sucrose. In this system, ABA could also induce anthocyanin synthesis without addition of high concentration of sucrose. The amount of endogenous ABA transiently increased just before the induction of anthocyanin synthesis by high concentration of sucrose. These results suggested that change in the amounts of endogenous ABA might play an important role in the induction of anthocyanin synthesis in the regenerated torenia shoots. In the previous study, we performed cDNA subtraction and obtained the genes up-regulated in the regenerated torenia shoots. In this study, gene expression profiles of 1200 clones obtained by cDNA subtraction were analyzed chronologically by cDNA microarray under the each condition of high concentration of sucrose or addition of endogenous ABA.
