Abstract
Aluminum (Al) resistance in wheat is primarily controlled by the ALMT1 protein Al-activated malate transporter which was localized on the plasma membrane. Several ALMT homologues were found in Arabidopsis, rice and other plant species, but not in animal and bacteria, indicating that the ALMT family consists of plant-specific transporter proteins. To elucidate the function and structure of wheatALMT1 protein, we have tried to purify the protein using heterologous expression systems as follows. The functional expression of the ALMT1 with a C-terminal 6-histidine tag was confirmed in tobacco cultured cells (BY-2). The ALMT1-His was also expressed in yeast or was synthesized using a wheat germ cell-free protein synthesis system. We will report partial purification of the protein.
