Abstract
Stomata surrounded by a pair of guard cells in the epidermis regulate gas exchange between plants and atmosphere. Stomata open through activation of plasma membrane H+-ATPase in guard cells in response to blue light. Under drought condition, stomata close through activation of plasma membrane anion channel in response to abscisic acid. In this study, to elucidate signaling pathways in response to blue light and abscisic acid, we screened stomatal aperture mutants by water loss in detached leaves from EMS-treated Arabidopsis. We isolated 2 mutants named std1 and std2 and 2 mutants named ftd1 and ftd2. std1 and std2 showed closed stomata under light illumination. In contrast, ftd1 and ftd2 showed open stomata even in the dark condition. we are trying to identify the FTD2 locus by map-based cloning. We'll report the results of mapping and analysis of detailed phenotype in ftd2.
