Expression analysis of the DAD1 and BI-1 in Arabidopsis

Abstract

Programmed cell death (PCD) plays an essential role to the development and maintenance of bodies. Dysfunction of DAD1 (Defender against Apoptotic cell Death) has been linked to PCD in animals and plants. One type of PCD in animals is triggered by cytochrome c release from mitochondria via pores formed by BAX proteins. This type of PCD can be prevented by expression of BAX inhibitor 1 (BI-1). In last report, the promotor-GUS assay had shown that the genes of AtDAD1 and AtBI-1 express the root cap, a vascular bundle of the root, an abscission zone of the silique and a septum of the maturity silique. These results suggest the involvement of the AtDAD1 and AtBI-1 in the developmental programmed cell death. In this report, we analyzed effect of stress on the activation of ATDAD1 and AtBI-1 in Arabidopsis T87 cultured cells.