Abstract
Phospholipid turnover is well-known as an adaptive response of eukaryotic cell to abiotic stress. In plant, phosphatidylcholine (PC) is the major phospholipid constitute of cell membrane and synthesized via CDP-choline pathway. The first step of CDP-choline pathway is catalyzed by choline kinase (CK). Since CK activity was increased in Arabidopsis plant by salt treatment, we obtained Arabidopsis T-DNA insertion lines of CK isogenes. Germination rate of At4g09760 mutant in MS medium supplemented with NaCl was decreased compared to wild type. When seedlings were grown with NaCl for 2 weeks after germination, fresh and dry weights were not changed between ck mutants and wild type. PC contents in shoot were comparable between ck mutant and wild type under a control condition, and not significantly changed after salt treatment. However, choline content in shoot of At1g74320 mutant was higher than that of wild type under a control condition.
