Abstract
Aluminum (Al) stress causes inhibitions of plant growth in acid soil and many genes change their expressions under the stress. Isolation of transcription factor (TF) related to an expression of Al inducible AtGST11 in Arabidopsis thaliana was performed to characterize Al-specific gene response mechanism in this study.
TF proteins which can bind to promoter of AtGST11 gene were isolated by one-hybrid method and bio-panning. Seven and three cDNA clones were obtained as candidates by these methods, respectively. DNA sequence analysis indicated that putative bZIP transcription factor, ethylene response element binding factor 2 and BEL1-like homeobox 4 were included in these clones. Full-length cDNA corresponding to each candidate was used to again one-hybrid analysis to confirm the reproducibility. Furthermore, RT-PCR was performed to determine their gene-expression patterns under Al stress.
Now, the candidates are also determined their DNA binding capability to promote of AtGST11 by gel-shift assay.
