Abstract
To understand the diversities of biological characteristics among the lineage of plants and animals, it is important to analyze the genomic information of intermediate organisms that contain both animal and plant functions. We focus a biflagellate green alga, Chlamydomonas reinhardtii as a model system. To collect the genomic resources, full-length cDNA enriched libraries and a genomic BAC library were constructed using mt- strain C-9. Full length cDNA libraries were generated by an oligo-capping method using mRNAs isolated from cells cultured in various environment stress conditions. Both ends of the cDNAs in total 107,970 reads were assembled with known mRNA sequences and the draft genome sequence (JGI v.4.0). The 3,149 clusters covered by full-length cDNAs were identified from the 13,641 predicted clusters. Both ends of 6,310 BAC clones, which represent 20X coverage of the genome, were also mapped on the JGI draft genome sequence. Data of these resources were integrated in the Chlamydomonas genome database.
