Abstract
We have identified the novel protein Glycine max PEROXISOMAL ADENINE NUCLEOTIDE CARRIER (PNC1) by proteomic analyses of peroxisomal membrane proteins using a blue native/SDS-PAGE technique combined with PMF method1). Functional integration of Gm PNC1 and an Arabidopsis orthologue, At PNC2, into intact Escherichia coli cells revealed ATP and ADP import activities. We investigated the physiological functions of PNC1 in peroxisomal metabolism by analyzing a transgenic Arabidopsis plant in which expressions of Arabidopsis orthologues, At PNC1 and 2 were suppressed using RNA interference. The knock down Arabidopsis plant suppressed the degradation of storage lipids during post-germinative growth and required sucrose for germination. These results suggest that PNC1 contributes to the transport of adenine nucleotides that are consumed by reactions that generate acyl-CoA for peroxisomal fatty acid β-oxidation during post-germinative growth.
1) Yuko Arai, Makoto Hayashi, and Mikio Nishimura (2008) The Plant Cell, in press.
