Abstract
Stability is a significant feature of recombinant protein production in transgenic plants. Preivously we have generated the transgenic tomato with miraculin gene that encodes a taste-modify protein (Sun et al., 2007). The miraculin gene is driven by CaMV35S promoter and terminated by nos terminator. We are optimizing effective and suitable environmental conditions for the miraculin production. Miraculin content and miraculin gene expression were decreased in high light intensity condition when cultivated in various light intensities. Moreover, the ratio of mRNA which read-through the termination region was increased in high light intensity condition. These results suggested that the ability of termination by nos terminator was affected by high light intensity and led to the reduction of miraculin accumulation in the transgenic tomato. Currently we are testing a new terminator for the ability of termination in strong light condition in newly generated transgenic tomato.
