Abstract
In Arabidopsis genome, about 1,400 transporter-related genes were fond in silico, of which function is almostly unknown. To overcome round robin screening of novel transporter genes from candidates, we prepared transorter-specific gene library Amethyst (Arabidopsis membrane transporter and hydrophobic protein cDNA clones stack).
In this study, we prepared over-expression library of Amethyst in mammalian culture cell. 961 genes were subcloned to CMV promoter driven mammalian expression vector. Then, as a test case, we screened molybdenum, one of a trace elements, related transporter. pCMV-Amethyst and molybdenum-sensing MolyProbe sensor protein were co-expressed in 293T culture cell, and molybdenum depending FRET pattern were analysed.
