Plant and Cell Physiology Supplement
Abstract of the Annual Meeting of JSPP 2010
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5' untranslated region analysis of NIP5;1, a boron deficiency inducible gene, in Arabidopsis thaliana
*Mayuki TanakaToru Fujiwara
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Pages 0064

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Abstract
NIP5;1, an aquaporin-like gene, is a boron (B) channel required for normal plant growth under low B conditions and its transcript accumulation is .upregulated under low B conditions (Takano et al., 2006). We have already reported that at least three distinct elements in NIP5;1 promoter regions were involved in the expression of three different portions of roots under low B condition and 5'untranslated region (5'UTR) (312 bp) is involved in NIP5;1 transcript accumulation in response to low B in last meeting.
To elucidate mechanisms of NIP5;1 regulation in response to B more detail, a series of truncated 5'UTR were generated, were fused to the GUS reporter gene under the control of cauliflower mosaic virus 35S RNA promoter and were introduced into Arabidopsis. The GUS expression of transgenic plants inserted 312 bp 5'UTR is higher in low B condition than in high B condition. On the other hand, the GUS expression of transgenic plants inserted 5'UTR but not including +184-+197(13bp) is high in both low and high B conditions. These results suggested that at least +184-+197 regions of 5'UTR are important for NIP5;1 transcript accumulation in response to low B.
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© 2010 by The Japanese Society of Plant Physiologists
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