Abstract
Phytoremediation is known as a tool to clean-up excess pollutants from the environment. To use plants more efficiency for phytoremediation, it is helpful to know how plants obtained the resistance against pollutants. In this study, we focus on one of water and/or soil pollutant, selenium (Se), and try to identify the gene participate in plant Se-resistance by QTL analysis. Recombinant inbred lines (RIL) were made from progenies from F1 hybrid between two Arabidopsis thaliana ecotypes (selenium resistant, Col-0 and selenium sensitive, Ws-2). Among 205 F7 RILs, we measured root length of selected 99 RILs grown with or without selenite. Simultaneously, we performed genotype analysis of each RIL using 33 SSLP or CAPS markers. QTL analysis of RILs showed a loci located on chromosome 4 (close to ciw7 marker) significantly involve in the resistance to Se. Moreover, semi-fine-mapping analysis of RILs indicate that genes involve in Se resistance is located between two markers, FCA8s and ciw7, on chromosome 4. Since there are about 450 candidate genes in the identified region, we hereafter carry out fine-mapping analysis at the region.
