Abstract
In Arabidopsis, myosin VIII class consists of four members: ATM1, ATM2, VIIIA and VIIIB. There were several reports that tail domains of myosin VIII were localized at plasmodesmata, cell nuclei, endoplasmic reticulum and endosomes, suggesting that tail domains were diversified among myosin VIII members and each of them could associate with specific proteins. To elucidate the mechanism for each myosin VIII, we investigated the subcellular localization of full length myosin VIII which were transiently expressed in protoplasts and cultured cells. Myosin ATM1 and VIIIA were localized at the plasmodesmata, and in dot-like structures. Myosin ATM2 located on the dispersed dots and in cell nuclei. Myosin VIIIB was localized in condensed membranous structures. Next, we searched proteins that specifically interact with myosin VIII tail domains by pull down analysis. We will discuss the function of candidate molecules as the adaptor proteins of myosin VIII members.
