Abstract
We have developed a methodology for extraction of promoter constituents based on distribution profile of short sequences (Yamamoto et al, BMC Genomics 8: 67 2007). One group of the extracted elements is named as Regulatory Element Group (REG) due to shared characteristics with known transcriptional regulatory elements. Because biological roles of considerable amount of REGs are not known, information of these functions is quite helpful for understanding plant promoters. In this article, we will report our recent activities for massive identification of transcriptional regulatory elements. Our analysis includes prediction of regulatory elements using private and public microarray data, construction of synthetic promoters driving a luciferase-based reporter gene, validation of in vivo function of the predicted elements. We hope our analysis will reveal function of REGs and identify novel regulatory elements that belong to non-REG type.
