Abstract
We show two analytical conditions for CE/MS analysis with a fused silica capillary column to profile anionic metabolites at high speed and in high resolution. The first one aimed simultaneous analysis of a number of major anionic compounds including organic acids, sugar phosphates, nucleotides and coenzymes. Under this condition where ammonium formate (pH 8.0) was used as electrolyte, a standard mixture including 35 compounds could be analyzed less than 17 minutes, indicating that this is a rapid method comparable with the conventional method with polymer coated capillary column. However, the peaks of pentose phosphate isomers and hexose phosphate isomers were overlapped under this analytical condition. Therefore, we also employed another analytical condition where a mixture of ammonium acetate (pH 10.0) and methanol was used as electrolyte. Under this analytical condition, peaks for isomers could be separately determined; e.g. R5P, Ru5P, F6P, G1P, G6P. Gal1P and M6P. Taken together with the results of analyses of Arabidopsis leaf extracts and extracts from moss, we will discuss how these analytical conditions are useful in precise profiling of anionic metabolites.
