Abstract
Metabolome analysis using mass spectrometry such as GC-MS or LC-MS has been developed to identify metabolites in plants with an improved efficiency. Recently, CE-MS is reported to be a useful tool to identify plant metabolites, especially the charged species.
In this study, we performed a simultaneous quantitative analysis of metabolites such as various primary metabolites including amino acids, organic acids and phosphate compounds in A. thaliana shoot and root at different growth stages by using CE-ESI-QTOF. We also attempted the quantification of plant hormones together with primary metabolites. In case of non-target analysis in positive mode, 548 and 604 peaks were found in shoot and root, respectively. Of these, 25 and 23 compounds in shoot and root respectively could be identified e.g. as amino acids. In negative mode, 199 and 214 peaks in shoot and root respectively were identified. Of these, 7 compounds in shoot and 10 compounds in root were identified; these included organic acids, phosphate compounds and others. Indole-3-acetic acid (IAA) was also identified simultaneously with primary metabolites.
