Integration of transcriptomics with metabolomics to uncover hormone- regulated metabolism in Arabidopsis

Abstract

Phytohormones are key players in regulation of plant growth, development and defense response. In this study, we conducted comprehensive analysis of phytohormones regulating metabolic pathways using integrated omics data collected from Arabidopsis mutants of basic phytohormones, Auxin, Cytokinin, Gibberellin, Abscisic acid, Ethylene, Jasmonic acid and Brassinosteroid.
We used 28 hormone mutants and 4 wild types to collect gene expression profiles and metabolome profiles. The seven-day-old seedling grown in 1/2 MS medium were analyzed by DNA microarray (Affymetrix ATH1 GeneChip) and by LC-Q-TOF/MS, GC-TOF/MS, LCMS-IT-TOF/MS and CE-TOF/MS. Omics profile of each mutant was characterized by Ontology analysis. Furthermore, gene expression responses and metabolite accumulations related to specific hormone mutants were identified. We also analyzed co-expression pattern between genes and metabolites to extract metabolites accumulated in hormone-related pathways. We will present relationships between phytohormone functions and metabolic pathways.