Abstract
CLE genes are the family of genes encoding signaling peptides in their C terminal regions homologous to CLAVATA3 (CLV3). CLE genes are thought to regulate the size of meristems and their differentiation. Previous findings suggested that CLE1-7 are expressed in the root. The purpose of this study is to clarify the roles of CLE1-7 in the root development in Arabidopsis.
Expression of CLE1, 3, and 7 was induced by low NO3- in the medium, and was repressed by high NO3-. CLE1, 2, 3, 4, and 7 was expressed in pericycle cells. Lateral root lengths of CLE1-7 overexpressors were shorter than that of the wild-type plants. CLE1-7 was suggested to act as repressors of lateral root elongation.
The clv1 mutants having mutations in CLV1, the receptor of CLV3, had longer lateral roots than the wild-type plants. Microscopic analysis of CLV1-GFP revealed that CLV1 was expressed in the phloem companion cells of root. These results suggested that the CLV1 act as a receptor of CLE1-7 signaling peptides. We will also show the results from the analysis of the root length of CLE3 overexpressor in clv1 mutants, and discuss about the relationships between CLE3 and CLV1.
