Abstract
In the development of multicellular organisms, there is believed to be a system that integrates cell proliferation and cell expansion. Compensation is a phenomenon in which a decrease in cell number seems to trigger an increase in cell size, and this suggests existence of such a system. We have reported on our analyses of five fugu mutants that exhibit compensation. Here, we report about reverse genetic analysis on fugu2 mutant. Microarray data followed by RT-PCR conformation indicated that the expression of 46 genes was enhanced and that of 39 was repressed more than three fold in fugu2 leaves. To analyze the function of these genes in leaf organogenesis, we isolated 27 homozygous T-DNA insertional lines for these genes, and analyzed their leaf phenotype. As a result, we found several lines that showed defects in cell proliferation. Now, we are constructing double mutants between fugu2 and these lines to analyze the function of these genes in compensation. In this presentation, integrating these results we will discuss the mechanism of compensation in fugu2 mutant.
