Abstract
Proteomics is one of the best available tools for studying posttranslational modifications (PTMs), and it has no limitation like those encountered with forward genetics. Therefore, it is well suited for the analysis of unknown signaling pathways. Among the several PTMs described thus far, phosphorylation is the most extensively studied, and they have been shown to play a role in plant immune signaling.
We have developed a phosphoproteomics platform, which enables monitoring phosphorylation events in plant cells at the cellular level 1). Using our approach, we have identified 5,143 and 6,919 unique phosphopeptides from unfractionated Arabidopsis and rice cell lysates, respectively.
Besides developing the phosphoproteomics platform, we have also established a method for the differential analysis of plant samples which enables proteome dynamics to be monitored. Currently, we are analyzing phosphorylation system which regulates plant immune signaling, by using the established differential phosphoproteomics platform.
1) Mol Syst Biol. 2008;4:193. Epub 2008 May 6.
