Abstract
We quantify the transcripts, proteins, metabolites etc. as relative quantification. Northern hybridization analysis quantifies the transcripts based on total amounts of RNA or mRNA. RT-PCR including real-time RT-PCR quantifies the transcripts based on the level of reference gene. DNA array assay detects the relative levels of transcripts among the samples by normalization. CBB-stained SDS-PAGE, immunoblot analysis and ELISA usually quantify a particular protein level as relative quantification based on the weight of total protein, fresh weight, dry weight or culture volume. Quantify of metabolites is often based on the weight of total protein, fresh weight or dry weight. It is difficult to know which base is no difference among the samples before analysis such as novel mutant or treatment analysis. The normalization using total RNA, total mRNA total protein, fresh weight, dry weight or culture volume has potentially the problem that those are fluctuating. We report the quantificational method based on copy number of genomic DNA that is comparatively stable in a cell.
