Abstract
A unicellular green alga, Chlamydomonas reinhardtii, induces a carbon-concentrating mechanism (CCM) under limiting-CO2 conditions in light. Low-CO2 inducible genes have been identified by using cDNA array (Yamano et al. 2008). One of the low-CO2 inducible proteins, LCI14, contains a CobW_C-domain and a WW-domain is predicted to be a transcriptional regulatory factor. The LCI14 homologue is found in the Arabidopsis genome and predicted to be a PRL1-interacting factor. Arabidopsis PRL1 is known to be a transcriptional factor regulating the expression of genes involved in glucose metabolism (Nemeth et al. 1998). In order to characterize the function of LCI14 in Chlamydomonas CCM, amiRNA (artificial microRNA) construct of Lci14 was introduced into wild type cells. In the construct, amiRNA-MAA7 and amiRNA-Lci14 precursors are driven by the HSP70A-RbcS2 (AR) promoter. 5-fluoroindole (5-FI) was used for screening of MAA7 knock down transformant. The expression of Lci14 and the phenotype under low-CO2 condition of the 5-FI resistant transformants will be presented.
