Abstract
Although dynamics of molecules or organelles in the local region of a cell can be detectable by a total reflection immunofluorescent microscopy or by a video microscopy, it is not easy to detect these local molecular/particle behaviors and the global cellular events simultaneously. In order to detect local cytoplasmic behaviors and global cellular events in parallel, we have developed a new microscope system. As our system has two optical paths, one equipped with EMCCD camera for high resolution imaging of local cytoplasmic/molecular dynamics, and another equipped with a confocal laser scanning microscope system (C1) for detecting global cellular events in the same cell. Using this system, we could record particle behaviors on the cortical division site and global cell events in parallel during the progression of the cell division of a Tradescantia stamen hair cell without exchanging objectives. As our system controls the positioning of Z-axis of a stage, we could record two different focus areas in parallel. We also show some results using other materials, and tentative problems and the potential application will be discussed. This work was supported by JST SENTAN.
