Abstract
The micronutrient zinc is essential for all organisms because it is required as a cofactor in over 300 enzymes. On the other hand, excess zinc is also toxic to cells, presumably due to competition with other biologically important ions, which in plants ultimately leads to reduced biomass leaf chlorosis, and root inhibition. To understand the effect of excess zinc on plant cells, we have performed iTRAQ analysis using microsomal fractions from Arabidopsis roots. Arabidopsis grown for 10 days on MS plate or MS plate supplemented with high concentration zinc (300μM ZnSO4), and microsomal fractions were isolated. Each fraction was used for iTRAQ analysis. About 1000 proteins including membrane proteins by zinc treatment were identified and quantified. The changed ratio of these proteins was calculated using software MASCOT. We would like to discuss mechanisms of zinc tolerance in Arabidopsis from the iTRAQ data.
