Isolation and analysis of defensin protein Bj-AFP1 from Brassica juncea

Abstract

Many living organisms produce small antimicrobial peptides to protect their tissues from infectious microbial agents. Plant defensins are small, highly stable, cysteine-rich antimicrobial proteins that are thought to constitute an important component of plant defense against fungal pathogens. There are a number of such defensins expressed in various plant tissues with differing antifungal activity and spectrum.
We tried to isolate defensin proteins from Brassica juncea seed. The isolated protein was same amino acid sequence with Rs-AFP1 from Raphanus sativus. So we named the protein Bj-AFP1.
In this study, we report the isolation of defensin protein Bj-AFP1 from the seed of Brassica juncea and measurement of anti-microbial assay to several microorganisms.