Abstract
Flowering and following seed production of Japanese beech tree (Fagus crenata) often fluctuate widely from year to year. We have isolated Arabidopsis orthologs, constans (CO) and flowering locus T (FT) genes from Japanese beech tree, which are mainly function in flower transition. In Tohoku Regional Office, there are 38 clones collected from seven prefectures including Niigata and Tohoku area and propagated by grafting. Their comparison of nucleotide sequences were performed between two of polycarpic and the other fluctuating lines. It seemed likely that there are no such nucleotide polymorphisms within ORF of CO gene that influence its protein function among these clones. We are trying to isolate genomic clones for further analysis, and their variation of mRNA amount during the seasonal changes are also investigated.
