Abstract
Positional information is crucial to determine cell fate, underlying basis of plant morphogenesis. Plasmodesmata constitutes one pathway of such information, especially for transport of macromolecules. It is widely believed that plasmodesmata adjust their own size exclusion limit (SEL) to control intercellular movement of the large and informational signals , and accordingly play important roles for plant development. To date, although spatio-temporal regulation of SEL has been proposed at a tissue level in several angiosperms, it remains largely unknown how the regulation of SEL influences developmental state of cells.
We, therefore, decided to take advantage of a simple body plan of protonemata of the moss, Physcomitrella patens to approach this issue. We made the transgenic lines that constitutively express the photoswitchable fluorescent protein Dendra2, and observed a movement of the protein at a cell level, analysing a relationship between the regulation of SEL and differentiation states of cells.
