Abstract
In higher plants, metabolism of sugar (C) and nitrogen (N) is mutually regulated. To clarify the C/N regulator, we isolated novel C/N regulatory gene ATL31. The ATL31 belongs to ubiquitin ligase ATL family, sharing ATL6 with most similarity to the amino acid alignment. Plants overexpressing the ATL31 and ATL6 showed insensitivity to C/N stress conditions, whereas the single knockout mutants did the hypersensitivity. To investigate the detailed functions of both genes, we have generated double knockout mutants (atl31-1 atl6-1). The atl31-1 atl6-1 developed the etiolated true leaves and the elongated primary root length under normal growth conditions. Now we are going to try to figure out the characterization of atl31-1 atl6-1 in terms of these phenotypes. How both genes contribute to C/N response regulation in plants will be discussed.
