Abstract
Jasmonates(JAs) are plant signaling molecules, which have crucial roles in developmental processes and defense responses against wounding and pathogens. In JA signaling pathway, JAZ family proteins are the repressors of MYC2, a JA responsive transcription factor. After the degradation of JAZs by SCFCOI1, MYC2 is released and regulates the expression of jasmonates-responsive genes. Although MYC2 is well known to interact with JAZ proteins, other transcription factors, which interact with JAZ proteins, are still unknown. To identify transcriptional regulators in JA signaling, we performed cluster analysis of JA responsive genes. Based on the results, we focused on a bHLH transcription factor and designated the protein as INU1. Here, we tested interactions between INU1 and JAZ proteins using yeast two-hybrid system. A few JAZ proteins were found to interact with INU1. Moreover, INU1 formed heteromeric dimers with some other bHLH proteins including MYC2. In this presentation, we will show phenotype of inu1 and related single and double knockout mutants in Arabidopsis.
