Abstract
We studied absorption and translocation manner of Mg using Oryza Sativa L. cv. Nipponbare and suggested the involvement of transport proteins in our previous report. In this study, the role of nine proteins in total were studied (OsMGTs). In the case of amino-acid sequence of OsMGTs, these candidates belonged to 2-TM-GxN type transporters, found in the CorA super-family proteins that distributed widely in nature. The ORF region of OsMGT was inserted to the plasmid, and was introduced to the yeast mutant lacking Mg transport activity. Then the yeast was grown on a plate medium or in culture solution containing different concentration of Mg. It was found that among the yeast tested, three OsMGTs showed the functional complementarity of Mg transport. There is a possibility that each OsMGT has different role(s) for Mg transport and homeostasis. It was reported that some transporters of CorA super-family have different cation selectivity. Therefore, the transport capability of other cations such as Co2+ is now under analysis.
