Abstract
The SE-CC complex plays the central role in sucrose translocation through the phloem. During the sink-to-source transition of leaves, the SE-CC complexes in the minor veins acquire the sucrose translocation capacity, which includes the secondary plasmodesma formation in the cell wall between SE and CC. We previously isolated rsx1 mutants that showed restricted sucrose translocation from a source leaf to shoots and displayed nascent secondary plasmodesmata in the cell wall between SE and CC. RSX1 is hypothesized to digest pectate in the middle lamella of the cell wall and promote the secondary plasmodesmata formation. However, the exact function of RSX1 in this process and the roll of RSX1 in the construction of sucrose translocation pathways in source leaves remain to be elucidated. In this report, we created transgenic RSX1-sGFP rsx1-2 plants that successfully recovered from the defects in the sucrose translocation capacity. The subcellular localization of RSX1-sGFP in the leaf veins of the transgenic plants will be reported.
