Abstract
In acidic soils, aluminum (Al) hydrolyzed into Al3+ cations and it become a limiting factor for plant productivity. Al resistance is an important agronomic trait for worldwide crop production. Previous research reported that the toxic effect of Al can reduced by chelating Al with organic anions such as oxalate, citrate, or malate. In wheat (Triticum aestivum), malate exudation is the major organic anion involved with Al resistance, and Al-activated malate transporter, ALMT1, was identified and characterized. Although QTL analyses suggested that Al resistance in wheat is multigenic, physiological evidence for multiple mechanisms of Al resistance has not yet been documented, including activation factors of ALMT1. In this report, we selected 2 wheat lines which show same genotype and expression levels of ALMT1. Several genes were isolated with cDNA subtractive method using these 2 lines. Chromosomal location of these genes was investigated, and expression analyses of these genes were performed with several wheat lines that show different Al sensitivity.
