Light-dependent interaction of peroxisomes with mitochondria and chloroplasts is regulated by photosynthesis

Abstract

Leaf peroxisomes participate in photorespiration with mitochondria and chloroplasts. Here, we have studied the interaction between peroxisomes with mitochondria and chloroplasts to elucidate the role of the interaction in metabolic pathway. The results showed that the interaction of peroxisomes with chloroplasts is enhanced under light condition with elongation of peroxisomes along chloroplasts. Further analyses with two-photon laser scanning microscopy showed that the interaction is regulated by blue and red light. Then, we investigated the effect of photosynthetic inhibitors, DCMU and DBMIB, on the interaction of peroxisomes with mitochondria and chloroplasts. The results showed that peroxisomes and mitochondria are both sphere and weaken the interaction with chloroplasts similar to that in dark condition. The interaction of peroxisomes with chloroplasts was lost in white sector of var2, indicating that the interaction between these organelles is regulated by photosynthesis. These findings suggest that the photosynthetic regulation of the interaction between these three organelles would be reasonable for efficient metabolite flow in photorespiration.