Abstract
The gene of Sll1558 in Synechocystis PCC 6803 was identified as up-regulated gene by microarray analysis under the acid stress condition (pH 3.0). Relative expression levels of the gene at 0.5, 1 and 4 hours were 3.51, 6.94 and 4.05 respectively. The gene encodes mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) which catalyses D-Mannose 1-phosphate to GDP-D-mannose and involves N-glycan biosynthesis.
In the Synechocystis genome, sll1496 was also annotated as the enzyme. In this study, deletion mutants of the genes were constructed. The deletion mutants of sll1558 were more sensitive to acid stress compared with wild type cells. These mutants also display increased sensitivity to salt (0.5M NaCl) and osmotic (0.5M sorbitol) stress. Real-time PCR analysis of the levels of the transcripts in the wild type cells and mutants will also be reported.
