Abstract
Rice was aseptically grown to obtain secreted proteins for comprehensive analysis. Rice plants were grown in a nutrient solution from 1 to 2 weeks after germination, then the culture solution was collected. After root sample was obtained, 400 ml nutrient solution was concentrated to about 100 μl. Sample was purified using 2-D clean-up kit then protein obtained from root and nutrient solution was compared using SDS-PAGE. The band pattern was completely different, thus we considered that those protein in the nutrient solution was selectively secreted from the root. Though we have applied MALDI-TOF-MS after 2D gel separation, only 4 proteins were identified. For the comprehensive analysis of secreted protein, we have applied LC-MS/MS based shotgun sequencing then found a large number of proteins which are similar to pathogen related proteins and so on.
