Abstract
NBRP Tomato focuses on the development of bio-resources using Micro-Tom, a model cultivar of tomato genomics. Micro-Tom is a member of Solanum lycopersicum whose genome draft sequence has been obtained using cultivar Heinz 1706. Analysis of EST- and BAC sequences demonstrated that a genome sequence of Micro-Tom has considerable number of polymorphisms compared to that of Heinz 1706 in single-nucleotide level. Thus, genome sequencing of Micro-Tom will facilitate the development of a number of DNA markers and accelerate the elucidation of genes responsible for the phenotypic changes by using Micro-Tom mutagenized lines.In this study, Kazusa DNA Research Institute and the National Institute of Genetics (NIG) collaborate in sequencing by using Roche GS-FLX and Illumina HiSeq, respectively. Since the end of Nov. 2010, GS-FLX reads have accumulated to 10-fold coverage, and HiSeq Reads have accumulated to 120-fold coverage of tomato genome (0.9 Gb). We will report an up-to-date situation of read mapping and contig assembly, and data opening schedule as well. This work was supported by NBRP genome upgrading program Micro-Tom Genome Sequencing.
